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NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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Related Experiment Video

Updated: Jul 1, 2026

An Orthotopic Sciatic Nerve Xenograft for Neurofibromatosis Type 1 Neurofibromas
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An Orthotopic Sciatic Nerve Xenograft for Neurofibromatosis Type 1 Neurofibromas

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New insights into NF-kappaB regulation and function.

Shao-Cong Sun1, Steven C Ley

  • 1Department of Immunology, MD Anderson Cancer Center, University of Texas, Houston, TX 77030, USA. ssun@mdanderson.org

Trends in Immunology
|September 9, 2008
PubMed
Summary

Nuclear factor-kappaB (NF-kappaB) regulates immune responses through protein modifications. Dynamic ubiquitination and deubiquitination events are crucial for both canonical and noncanonical NF-kappaB signaling pathways.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Signaling

Background:

  • Nuclear factor-kappaB (NF-kappaB) transcription factors are key regulators of immune responses.
  • NF-kappaB proteins are typically held inactive in the cytoplasm by inhibitory proteins (IkappaB).
  • Activation involves IkappaB kinase (IKK)-mediated phosphorylation, ubiquitination, and degradation of IkappaB, allowing NF-kappaB nuclear translocation.

Purpose of the Study:

  • To highlight the critical roles of dynamic ubiquitination and deubiquitination in NF-kappaB signaling.
  • To explore the involvement of ubiquitination in the noncanonical NF-kappaB pathway.
  • To identify novel regulators of nuclear NF-kappaB transcriptional activity.

Main Methods:

  • Analysis of canonical NF-kappaB pathway activation via IKK, IkappaB phosphorylation, and proteasomal degradation.
  • Investigation of ubiquitination's role in the noncanonical NF-kappaB pathway, including p100 processing.
  • Identification and characterization of novel proteins influencing nuclear NF-kappaB activity.

Main Results:

  • Dynamic ubiquitination-deubiquitination events are essential for regulating the canonical NF-kappaB pathway.
  • Ubiquitination is critical for activating the noncanonical NF-kappaB pathway.
  • Novel regulatory proteins that control the transcriptional activity of nuclear NF-kappaB have been identified.

Conclusions:

  • Ubiquitination and deubiquitination are central to NF-kappaB pathway regulation.
  • Understanding these post-translational modifications provides insights into immune response mechanisms.
  • New research expands the knowledge of NF-kappaB pathway complexity and its regulators.