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Peritoneal morphological and functional changes associated with platelet-derived growth factor B.

Davide Cina¹, Pranali Patel, Jessica C Bethune

¹Department of Medicine, McMaster University, Hamilton, ON, Canada.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association

|September 9, 2008

Summary

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Platelet-derived growth factor (PDGF) induced blood vessel growth in the peritoneum but did not cause fibrosis or significant ultrafiltration issues. PDGF-B may contribute to peritoneal dialysis complications but is not the sole factor.

Area of Science:

Nephrology
Cell Biology
Tissue Engineering

Background:

Long-term peritoneal dialysis (PD) can cause peritoneal membrane changes like angiogenesis, fibrosis, and epithelial-mesenchymal transition.
Platelet-derived growth factor (PDGF) is linked to these injury responses.

Purpose of the Study:

To investigate the role of PDGF-B in inducing morphological and functional changes in the rat peritoneal membrane.
To assess if PDGF-B contributes to fibrosis and epithelial-mesenchymal transition relevant to PD complications.

Main Methods:

Rats received intraperitoneal injections of an adenoviral vector expressing PDGF-B.
Peritoneal membrane structure and function were analyzed post-sacrifice.
Gene expression related to epithelial-mesenchymal transition was assessed.

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Main Results:

PDGF-B overexpression led to significant angiogenesis, cell proliferation, and submesothelial thickening.
While transforming growth factor beta expression increased, its activation was limited, preventing significant collagen accumulation.
Increased solute transport was observed, but ultrafiltration function was only transiently and insignificantly impacted.
No evidence of epithelial-mesenchymal transition was found, and mesothelial cells showed increased collagen gene expression.

Conclusions:

PDGF-B induces peritoneal angiogenesis without causing fibrosis.
The absence of significant ultrafiltration dysfunction and epithelial-mesenchymal transition suggests PDGF-B is not the sole driver of PD-related peritoneal injury.