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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
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Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis.

María Florencia Del Papa1, Marta Perego

  • 1Division of Cellular Biology, Mail Code MEM-116, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Journal of Bacteriology
|September 9, 2008
PubMed
Summary
This summary is machine-generated.

Enterococcus faecalis utilizes ethanolamine, an abundant intestinal compound, as a nutrient source. This ability is controlled by the RR-HK17 two-component system (TCS), crucial for bacterial survival and colonization.

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Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Signal Transduction

Background:

  • Enterococcus faecalis is a Gram-positive bacterium found in the human gut.
  • Antibiotic resistance complicates treatment of E. faecalis infections.
  • Two-component systems (TCS) mediate bacterial adaptation and colonization.

Purpose of the Study:

  • To investigate the role of TCS in E. faecalis utilization of ethanolamine.
  • To identify the specific TCS involved in ethanolamine metabolism.

Main Methods:

  • Generating a response regulator RR17 mutant strain of E. faecalis.
  • Assessing bacterial growth in a synthetic medium with ethanolamine.
  • Measuring gene transcription and protein autophosphorylation in response to ethanolamine.

Main Results:

  • E. faecalis growth on ethanolamine requires the RR-HK17 TCS.
  • Ethanolamine induces transcription of the RR17 response regulator gene.
  • Ethanolamine directly activates the HK17 sensor histidine kinase.

Conclusions:

  • The RR-HK17 TCS regulates E. faecalis utilization of ethanolamine.
  • This TCS enables E. faecalis to adapt to nutritional conditions in the host intestine.
  • Understanding this pathway is key to controlling enterococcal colonization and infection.