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Acute phase protein response in goats.

Félix H D González1, Fernando Tecles, Silvia Martínez-Subiela

  • 1Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|September 9, 2008
PubMed
Summary

This study validated methods for measuring goat acute phase proteins (APPs) like haptoglobin and serum amyloid A. Inflammatory stimulus increased Hp, SAA, ASG, and fibrinogen while decreasing albumin.

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Area of Science:

  • Veterinary Medicine
  • Biochemistry
  • Animal Science

Background:

  • Acute phase proteins (APPs) are crucial biomarkers for detecting inflammatory conditions in animals.
  • Accurate measurement of APPs in goats is essential for diagnosing and managing inflammatory diseases.

Purpose of the Study:

  • To validate analytical methods for quantifying key APPs (haptoglobin, serum amyloid A, acid soluble glycoprotein, fibrinogen, albumin) in goats.
  • To assess the response of these APPs to an induced inflammatory stimulus in goats.

Main Methods:

  • Validation of assays for haptoglobin (Hp), serum amyloid A (SAA), acid soluble glycoprotein (ASG), fibrinogen, and albumin.
  • Induction of inflammation via subcutaneous turpentine oil injection.
  • Measurement of APP concentrations using validated methods.

Main Results:

  • Assays demonstrated good precision with low intra- and interassay coefficients of variation (CVs).
  • Linearity was observed for most APP assays across different sample dilutions.
  • Turpentine-induced inflammation significantly increased Hp, SAA, ASG, and fibrinogen levels.
  • Albumin concentrations showed a significant decrease following the inflammatory stimulus.

Conclusions:

  • Validated assays provide reliable quantification of APPs in goats.
  • Hp, SAA, ASG, and fibrinogen serve as sensitive indicators of inflammation in goats.
  • Albumin can be used as a negative APP marker in goats.