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Infinium Assay for Large-scale SNP Genotyping Applications
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Published on: November 19, 2013

New eximer-based tandem systems for SNP detection.

Daria S Novopashina1, Maria I Meschaninova, Svetlana A Kholodar

  • 1Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev ave., Novosibirsk 630090, Russia. danov@niboch.nsc.ru

Nucleic Acids Symposium Series (2004)
|September 9, 2008
PubMed
Summary

New pyrene-labeled oligonucleotide probes show promise for single nucleotide polymorphism (SNP) detection. These probes utilize fluorescence changes upon binding to DNA targets, enabling accurate genetic analysis.

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Area of Science:

  • Oligonucleotide chemistry
  • Molecular diagnostics
  • Biophysical chemistry

Background:

  • Single nucleotide polymorphisms (SNPs) are key genetic variations influencing disease risk and drug response.
  • Oligonucleotide-based probes are crucial tools for genetic analysis and diagnostics.
  • Pyrene-based fluorescent probes offer sensitive detection capabilities.

Purpose of the Study:

  • To design and synthesize novel pyrene-labeled oligo(2'-O-methylribonucleotide) tandems for SNP detection.
  • To investigate the utility of these probes in a fluorescence-based detection strategy.
  • To evaluate the potential of these probes for genetic analysis applications.

Main Methods:

  • Synthesis of mono- and bis-pyrene-labeled oligo(2'-O-methylribonucleotide) tandems.
  • Utilizing pyrene excimer formation as a detection signal.
  • Employing duplex melting curve analysis with excimer fluorescence registration.

Main Results:

  • Successful synthesis of novel pyrene-labeled oligonucleotide tandems.
  • Demonstrated SNP detection capability based on pyrene excimer fluorescence.
  • Validated the use of duplex melting curve analysis for excimer fluorescence-based detection.

Conclusions:

  • Pyrene-labeled oligo(2'-O-methylribonucleotide) tandems are effective probes for SNP detection.
  • The fluorescence-based detection strategy utilizing pyrene excimer formation is viable.
  • These probes hold potential for advanced genetic analysis and diagnostic applications.