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Updated: Jul 1, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Published on: July 28, 2017

A convenient approach towards 2'-O-modified RNA-oligonucleotides on solid support using universal nucleosides.

Jens Haas¹, Joachim W Engels

¹Johann Wolfgang Goethe-University Frankfurt, Department of Chemistry, D-60438 Frankfurt am Main, Germany.

Nucleic Acids Symposium Series (2004)

|September 9, 2008

Summary

Researchers developed a new method for modifying RNA oligonucleotides on solid supports. This efficient technique utilizes universal nucleosides with a 2'–O-aminopropyl–tether for improved synthesis.

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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Area of Science:

Chemical Synthesis
Oligonucleotide Chemistry
Biotechnology

Background:

Solid-phase synthesis is crucial for producing RNA oligonucleotides.
Modifications enhance RNA functionality but can be challenging to implement.
A need exists for efficient and versatile modification strategies.

Purpose of the Study:

To introduce a novel synthetic pathway for RNA oligonucleotide modification.
To demonstrate the utility of universal nucleosides with a specific tether for solid-phase synthesis.
To enable efficient and versatile modifications of RNA.

Main Methods:

Development of a novel synthetic route for RNA modification.
Utilizing universal nucleosides functionalized with a 2 -O-aminopropyl-tether.
Performing modifications on solid-support.

Main Results:

An efficient synthetic pathway for RNA oligonucleotide modification was established.
The 2 -O-aminopropyl-tether proved effective for solid-phase synthesis.
The method allows for versatile and efficient modification of RNA.

Conclusions:

The reported method offers an efficient approach for RNA oligonucleotide modification.
This strategy facilitates the synthesis of modified RNA with potential applications in therapeutics and diagnostics.
The use of universal nucleosides with the 2 -O-aminopropyl-tether represents a significant advancement in oligonucleotide chemistry.