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Updated: Jul 1, 2026

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
Published on: March 11, 2021
Near-infrared fluorescence detection of ATP-biotin-mediated phosphoprotein labeling
Xiaofei Gao1, Amy Schutz-Geschwender, Philip R Hardwidge
1Center for Infectious Disease Research and Vaccinology, South Dakota State University, Box 2175, 1119 North Campus Drive, Brookings, SD 57007, USA.
Abstract:
Detection of phosphoproteins plays an important role in understanding protein function in cellular signalling pathways. Improved methods for identification and quantification of phosphoproteins are research priorities. Near-infrared (NIR) fluorescence detection of a gamma-modified ATP-biotin analog was used to detect protein phosphorylation, using both model kinase substrates and mammalian cell lysates. NIR signal intensity was dependent on substrate and ATP-biotin concentrations.
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