Lipid imaging with cluster time-of-flight secondary ion mass spectrometry
Alain Brunelle1, Olivier Laprévote
1CNRS, Institut de Chimie des Substances Naturelles, Laboratoire de Spectrométrie de Masse, UPR 2301, Av. de la Terrasse, 91198, Gif-sur-Yvette, France. Alain.Brunelle@icsn.cnrs-gif.fr
Abstract:
This brief article provides an overview of the current state of the art in biological imaging mass spectrometry using cluster time-of-flight secondary ion mass spectrometry (TOF-SIMS). Recent and spectacular improvements in terms of sensitivity of TOF-SIMS imaging methods have allowed many biological applications to recently be successfully tested, such as mapping of lipid disorders in human muscles of a patient suffering from dystrophy, localization of surfactins after the swarming of bacteria on a surface, or lipid mapping over whole-body animal sections.
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