Histo-morphometric analysis applied to spleens of Marek's disease virus inoculated chickens

As part of a larger experiment to compare the pathogenic effects of different strains of Marek's disease virus, a counting procedure for tissue components in histological sections is presented which may quantify the relative volumes occupied by the components. The method is demonstrated on spleens taken from 8-weeks-old chickens, treated at one-day-old with a cell-associated pathogenic strain of Marek's disease virus (MDV), a cell-associated vaccine strain, or a MDV-free cell suspension.