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Related Concept Videos

Drug Dependence01:17

Drug Dependence

Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
Drug Abuse and Addiction: Pharmacological Phenomena01:15

Drug Abuse and Addiction: Pharmacological Phenomena

Drug dependence, abuse, and addiction are complex phenomena that can precipitate various abnormal states. Physical dependence refers to a state of pharmacological adaptation to a drug. This adaptation often results in tolerance—a reduced response to the drug after repeated administrations. When the drug use is abruptly stopped, withdrawal symptoms occur due to the body's need to readjust from the pharmacologically induced imbalance. However, tolerance and withdrawal symptoms do not necessarily...
Drugs Affecting Neurotransmitter Synthesis01:29

Drugs Affecting Neurotransmitter Synthesis

Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase, which converts...
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jul 1, 2026

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
09:54

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Published on: March 8, 2020

[Gene expression profiling in drug addicted brain].

Feng Chen1, Tao Li, Shuan-Liang Fan

  • 1Department of Forensic Sciences, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China. chenfeng418@stu.xjtu.edu.cn

Yi Chuan = Hereditas
|September 10, 2008
PubMed
Summary
This summary is machine-generated.

Drug addiction is a complex brain disease. High-throughput genomics and transcriptomics help elucidate the molecular mechanisms underlying addiction by analyzing gene expression profiles in the brain.

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Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
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Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods

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Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
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Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

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Last Updated: Jul 1, 2026

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
09:54

Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

Published on: March 8, 2020

Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods
09:29

Investigating Drivers of Antireward in Addiction Behavior with Anatomically Specific Single-Cell Gene Expression Methods

Published on: August 4, 2022

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
08:14

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

Published on: August 26, 2014

Area of Science:

  • Neuroscience
  • Genomics
  • Transcriptomics

Context:

  • Drug addiction is a chronic brain disease influenced by genetic factors and drug exposure.
  • Understanding the molecular mechanisms of addiction is crucial for developing effective treatments.
  • High-throughput technologies offer powerful tools for studying addiction biology.

Purpose:

  • To summarize the progress and applications of Serial Analysis of Gene Expression (SAGE) and microarray technologies in drug addiction research.
  • To highlight the utility of gene expression profiling in understanding addiction.
  • To provide an overview of how these technologies contribute to elucidating addiction's molecular basis.

Summary:

  • Serial Analysis of Gene Expression (SAGE) and microarray technologies enable comprehensive analysis of gene expression profiles in the brain.
  • These high-throughput methods are instrumental in identifying drug-induced molecular changes associated with addiction.
  • The study reviews the advancements and applications of SAGE and microarrays in the context of drug addiction.

Impact:

  • Facilitates a deeper understanding of the genetic and molecular underpinnings of drug addiction.
  • Provides a foundation for identifying novel therapeutic targets for addiction treatment.
  • Advances the field of addiction research by showcasing the power of transcriptomic approaches.