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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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[Identification of SNPs located in putative microRNA tar-get region of six functional genes in chickens through

Li-Ying Geng1, Chuan-Sheng Zhang, Li-Xin DU

  • 1Department of Life Science, Hebei Normal University of Science & Technology, Changli 066600, China. rosegengly@126.com

Yi Chuan = Hereditas
|September 10, 2008
PubMed

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...