[The development and application of method for detecting bovine complex vertebral malformation].

Area of Science:

• Genetics
• Animal Breeding

Context:

• Complex vertebral malformation (CVM) is a lethal autosomal recessive defect impacting Holstein calves.
• The genetic basis of CVM involves a G-to-T substitution in the solute carrier family 35 member 3 (SLC35A3) gene, crucial for UDP-N-acetylglucosamine transport.
• CVM is characterized by stillbirths, abortions, and premature births in affected calves.

Purpose:

• To determine the carrier frequency of the CVM mutation in a Chinese Holstein cattle population.
• To evaluate the efficacy of Created Restriction Site PCR (CRS-PCR) and Allele-specific PCR (AS-PCR) for CVM screening.

Summary:

• Genotyping of 436 Holstein cows and 93 Holstein bulls was performed using CRS-PCR and AS-PCR.
• Three carriers were identified: one cow and two bulls.
• Estimated CVM carrier frequencies were 0.60% in the Holstein dairy cattle population and 2.20% in the Holstein bull population.

Impact:

• Provides a reliable method for extensive CVM screening in Holstein cattle.
• Offers a theoretical foundation for the molecular diagnosis of CVM in Holstein calves.
• Contributes to genetic management strategies aimed at reducing the incidence of this lethal defect in cattle populations.