Related Experiment Video
Updated: Jul 1, 2026
08:40
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Computerized whole slide quantification shows increased microvascular density in pT2 prostate cancer as compared to
Cornelis G van Niekerk1, Jeroen A W M van der Laak, M Elisa Börger
1Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
The Prostate
|September 10, 2008
Summary
Prostate cancer (pT2) shows significantly increased microvascularity compared to normal tissue. This study quantifies increased vascular profiles (MVD), vascular area (MVA), and perfused surface area (MVP) in tumors.
Area of Science:
- Urology
- Oncology
- Pathology
Background:
- Contrast-enhanced imaging is crucial for prostate cancer detection and staging.
- Increased contrast agent uptake in prostate cancer suggests altered tissue physiology.
- Investigating microvascularity in pathology specimens can reveal underlying mechanisms.
Purpose of the Study:
- To determine if microvascularity is increased in prostate confined cancer (pT2).
Main Methods:
- Analyzed radical prostatectomy specimens from 26 patients with organ-confined tumors.
- Visualized microvessels using CD31 immunohistochemistry and automated scanning microscopy.
- Quantified microvessel density (MVD), vascular area (MVA), and perfused surface area (MVP) in tumor and normal prostate tissue.
Main Results:
- Tumor tissue showed significant increases in mean MVD (81%), mean MVA (49%), and mean MVP (62%) compared to normal tissue (P < 0.001).
- Tumor vasculature exhibited significantly decreased heterogeneity compared to normal prostate tissue (P < 0.001).
Conclusions:
- Microvasculature characteristics significantly differ between pT2 prostate tumors and normal prostate tissue.
- Findings support the role of altered microvasculature in early prostate cancer progression.
