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Updated: Jul 1, 2026
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Determination of propafenone hydrochloride by flow-injection analysis coupled with resonance light scattering
Xiaoli Hu1, Dongpo Xu, Shaopu Liu
1School of Chemistry and Chemical Engineering, Southwest University, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Chongqing 400715, People's Republic of China. xiaolihu@swu.edu.cn
Abstract:
A simple, sensitive and rapid flow injection analysis (FIA) method with resonance light scattering (RLS) was described for the determination of propafenone (PPF). The method was based on the ion-association reaction of 12-tungstophosphoric acid (TP) with propafenone. In pH 1.0 acidic medium, TP reacted with PPF to form an ion-associate complex, which resulted in a significant enhancement of RLS intensity. The maximum scattering peak was located at 340 nm, the RLS intensity was proportional to the concentration of PPF in the range 0.003-9.0 microg/mL, and the detection limit (3sigma) of 1.0 ng/mL was obtained at a sampling rate of 60 samples/h. The feasible reaction conditions and FIA parameters for the system were optimized. The method proposed in this paper shows satisfactory reproducibility with a relative standard deviation (RSD) of 2.1% for 10 successive determinations of 2.0 microg/mL PPF. The present method had been successfully applied to the determination of PPF in serum samples and pharmaceutical samples. The results obtained were in agreement with the method used in the Chinese Pharmacopoeia.
