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Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...

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Related Experiment Video

Updated: Jul 1, 2026

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
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A protein interaction network analysis for yeast integral membrane protein.

Ming-Guang Shi1, De-Shuang Huang, Xue-Ling Li

  • 1Intelligent Computing Lab, Hefei Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei Anhui, 230031, China.

Protein and Peptide Letters
|September 11, 2008
PubMed
Summary

Researchers identified 300 protein-protein interactions for 189 membrane proteins in Saccharomyces cerevisiae, revealing a highly connected yeast interactome network and its global topological features.

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Area of Science:

  • Molecular Biology
  • Systems Biology
  • Bioinformatics

Background:

  • Saccharomyces cerevisiae is a model single-celled eukaryote.
  • The protein-protein interactions of its integral membrane proteins are largely uncharacterized experimentally.

Purpose of the Study:

  • To identify and characterize protein-protein interactions of integral membrane proteins in Saccharomyces cerevisiae.
  • To analyze the global topological features and robustness of the yeast interactome network.

Main Methods:

  • Employed Greedy Kernel Principal Component analysis plus Linear Discriminant Analysis (a kernel method).
  • Identified protein-protein interactions and constructed a network.

Main Results:

  • Discovered 300 protein-protein interactions involving 189 integral membrane proteins.
  • Characterized a highly connected yeast protein-protein interaction network.
  • Studied the global topological features and robustness of the integral membrane protein network.

Conclusions:

  • Provides a comprehensive description of protein-protein interactions for yeast integral membrane proteins.
  • Reveals the global topology and system-level robustness of the yeast interactome.
  • Advances the understanding of yeast protein interactions.