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Updated: Jul 1, 2026

Myo-mechanical Analysis of Isolated Skeletal Muscle
Published on: February 22, 2011
Real-time polymerase chain reaction to follow the response of muscle to training
Lauren M Moore1, Andrew G Fisher, Judy M Coulson
1Department of Human Anatomy and Cell Biology, School of Biomedical Sciences, the University of Liverpool, Liverpool, UK.
Abstract:
The adaptive response of muscle to changes in activity or loading can take many weeks. Changes in the levels of RNA within a muscle fiber can give an early indication of the nature of the response of that fiber to changes in activity or loading. We have designed a new primer set for quantitative polymerase chain reaction (PCR) that will allow us to follow these early transcriptional changes in rat muscle, and have shown that analysis can be performed by standard techniques on as little as 5 mg of muscle, an amount that can be obtained by needle biopsy.
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