Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 1, 2026

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

A novel tissue engineering-based assay for immunological infertility.

G Ahangari1, H Naderimanesh, A Hossein-Nezhad

  • 1Department of Molecular Medicine and Immunology, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran. ghah@nigeb.ac.ir

Scandinavian Journal of Immunology
|September 11, 2008
PubMed
Summary

A novel tissue engineering method using collagen gel to detect antisperm antibodies (ASA) offers improved specificity and sensitivity for diagnosing immunological infertility. This approach shows promise for detecting autoantibodies in autoimmune diseases and monitoring bio-drug treatments.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple fractures in infants who have Ehlers-Danlos/hypermobility syndrome and or vitamin D deficiency: A case series of 72 infants whose parents were accused of child abuse and neglect.

Dermato-endocrinology·2018
Same author

The relationship between obesity and bone mineral density: evidence for a role of peroxisome proliferator-activated receptor gamma.

Minerva endocrinologica·2015
Same author

Association between alterations in global DNA methylation and predisposing factors in diabetes: a high pressure liquid chromatography based study.

Minerva medica·2014
Same author

Insulin resistance via modification of PGC1α function identifying a possible preventive role of vitamin D analogues in chronic inflammatory state of obesity. A double blind clinical trial study.

Minerva medica·2014
Same author

A polymorphism in the resistin gene promoter and the risk of multiple sclerosis.

Minerva medica·2013
Same author

A novel report of apoptosis in human lung carcinoma cells using selective agonist of D2-like dopamine receptors: a new approach for the treatment of human non-small cell lung cancer.

International journal of immunopathology and pharmacology·2013

Area of Science:

  • Reproductive Immunology
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Immunological infertility affects 9-36% of infertile couples due to antisperm antibodies (ASA).
  • Existing ASA detection methods lack reliability in distinguishing between causative and non-causative antibodies.
  • Variability in ASA specificity and biological effects complicates accurate diagnosis.

Purpose of the Study:

  • To develop a reliable and reproducible assay for detecting sperm antibodies.
  • To improve the specificity and sensitivity of ASA detection compared to conventional methods.
  • To explore the application of tissue engineering in immunological diagnostics.

Main Methods:

  • Utilized collagen gel as a biocompatible bio-scaffold for creating engineered sperm analogues.

More Related Videos

Establishment of an Embryo Implantation Model In Vitro
05:13

Establishment of an Embryo Implantation Model In Vitro

Published on: June 21, 2024

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis
07:46

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Published on: October 13, 2023

Related Experiment Videos

Last Updated: Jul 1, 2026

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

Establishment of an Embryo Implantation Model In Vitro
05:13

Establishment of an Embryo Implantation Model In Vitro

Published on: June 21, 2024

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis
07:46

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis

Published on: October 13, 2023

  • Developed a novel tissue engineering-based assay for ASA detection.
  • Compared the performance of the new assay against a conventional ASA detection test.

    • Main Results:

    • The tissue engineering-based assay demonstrated higher specificity and sensitivity than the conventional test.
    • The novel assay exhibited low intra- and inter-assay variations, indicating reproducibility.
    • Collagen gels offered advantages of biocompatibility, ease of fabrication, and low cost.

    Conclusions:

    • Tissue engineering offers a promising approach for developing more accurate diagnostic tools for immunological infertility.
    • The developed assay can reliably detect sperm antibodies contributing to infertility.
    • This methodology holds potential for detecting autoantibodies in autoimmune diseases and monitoring bio-drug therapies.