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[Preparation of the polypeptide antibody against human transcriptional factors TFDP1 and TFDP3]
Chan Tian1, Yun-yan Li, Huan Qiao
1Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.
Aim:
To prepare the polyclonal antibody against human transcriptional factors DP3(TFDP3) and DP1(TFDP1) and identify their reactivity and specificity.
Methods:
Two totally different peptides between TFDP1 and TFDP3 were chosen as antigens to inoculate rabbits. The proteins from HEK293 cells were transiently transfcted with eukaryotic expression vectors of TFDP1 and TFDP3. The reactivity and specificity of antibodies were detected by Western blot.
Results:
The amino acid residues of TFDP3 17-26 and TFDP1 17-32 were selected and synthesized to produce polypeptide antibodies. Western blot analysis demonstrated that anti-TFDP3 antibody could specifically recognize TFDP3 protein, while anti-TFDP1 antibody could both recognize TFDP1 and bind TFDP3 protein.
Conclusion:
The polypeptide antibodies against TFDP3 and TFDP1 have been prepared. The anti-TFDP3 antibody has no cross-reaction with TFDP1.
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