Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute Lymphoblastic Leukemia Presenting With Severe Acute Kidney Injury Requiring Hemodialysis due to Kidney Infiltration: A Case Report.

Clinical case reports·2026
Same author

Metabolic rewiring for enhanced itaconate yield through heterotrophic CO<sub>2</sub> fixation in Escherichia coli.

Journal of biological engineering·2026
Same author

Genome-Scale Engineering Importing Property of Escherichia coli for Improving Production of 3-Hydroxypropionic Acid.

Microbial biotechnology·2026
Same author

Focused Ultrasound-Triggered Burst Release and Enrichment of Engineered Bacteria for Tumor Therapy.

Biomaterials research·2026
Same author

A human-relevant epithelial-fibroblast co-culture platform for integrated analysis of PHMG-HCl-induced pulmonary fibrogenic responses.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same author

Bacterial conjugation and its deployment for diverse biotechnology applications.

Biotechnology advances·2026

Related Experiment Video

Updated: Jul 1, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

A two-step quantitative pathogen detection system based on capillary electrophoresis.

Gi Won Shin1, Yang Sook Cho, Hee Sung Hwang

  • 1School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.

Analytical Biochemistry
|September 11, 2008
PubMed
Summary

This study presents a novel two-step method for bacterial pathogen detection using multiplex polymerase chain reaction (PCR) and capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) analysis. The technique enables sensitive identification and quantification of multiple pathogens simultaneously, improving early infection diagnosis.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Related Experiment Videos

Last Updated: Jul 1, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Area of Science:

  • Microbiology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Rapid bacterial pathogen identification is crucial for effective patient management and timely antibiotic treatment.
  • Capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) coupled with polymerase chain reaction (PCR) offers high sensitivity, resolution, and reproducibility for pathogen detection.
  • Optimizing multiplex PCR primer design and conditions is essential for simultaneous analysis of multiple pathogens in a single assay.

Purpose of the Study:

  • To develop and validate a novel two-step CE-SSCP based technique for simultaneous detection and quantification of bacterial pathogens.
  • To optimize PCR primer design and multiplex PCR conditions for enhanced pathogen analysis.
  • To assess the sensitivity and clinical applicability of the developed method for identifying multiple bacterial strains.

Main Methods:

  • A two-step approach combining multiplex PCR for pathogen detection and singleplex PCR for quantification, both analyzed by CE-SSCP.
  • Design of specific PCR primers for optimal CE-SSCP separation based on molecular weight.
  • Optimization of PCR conditions for multiplex analysis of multiple target pathogens.
  • Quantification of individual pathogens using specific PCR primers and CE-SSCP analysis.

Main Results:

  • Simultaneous identification of eight clinically important bacterial strains using the optimized multiplex PCR and CE-SSCP method.
  • Accurate quantification of each identified pathogen down to a sensitivity level of tens of cells per milliliter.
  • Demonstration of the method's capability for sensitive and reproducible pathogen detection and quantification.

Conclusions:

  • The described two-step CE-SSCP based method enables sensitive pathogen detection via multiplex PCR and precise quantification via specific PCR.
  • This technique holds significant potential for clinical applications requiring rapid and accurate identification of multiple bacterial pathogens.
  • The optimized approach advances molecular diagnostic capabilities for infectious disease management.