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Related Experiment Videos

Silver tolerance and accumulation in yeasts.

M Kierans1, A M Staines, H Bennett

  • 1Department of Biological Sciences, University of Dundee, UK.

Biology of Metals
|January 1, 1991
PubMed
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Certain yeast strains exhibit tolerance to silver ions (Ag+) by accumulating silver nanoparticles (Ag0). Light exposure influences silver toxicity, with reduced silver species being less harmful to yeast growth.

Area of Science:

  • Microbiology
  • Biotechnology
  • Environmental Science

Background:

  • Silver ions (Ag+) are known for their antimicrobial properties, posing a toxicity challenge for microbial applications.
  • Understanding microbial tolerance mechanisms to heavy metals like silver is crucial for developing novel biotechnological strategies.

Purpose of the Study:

  • To investigate the tolerance of various yeast species to silver ions (Ag+) and explore the underlying mechanisms.
  • To assess the impact of light exposure on silver ion toxicity and yeast growth.

Main Methods:

  • Culturing of yeast strains (Debaryomyces hansenii, Candida albicans, Saccharomyces cerevisiae, Rhodotorula rubra, Aureobasidium pullulans) on solid media with varying AgNO3 concentrations.
  • Exposure of Ag-containing plates to light before inoculation.

Related Experiment Videos

  • Microscopic and spectroscopic analyses (electron microscopy, X-ray microprobe analysis) to characterize silver accumulation and speciation.

    • Main Results:

    • Yeast growth was observed at Ag+ concentrations up to 1-2 mM, with increased tolerance upon subculturing at higher concentrations (up to 5 mM).
    • Light exposure reduced Ag+ toxicity, indicating light-mediated reduction to less toxic silver species (Ag0).
    • Yeast colonies and surrounding agar blackened, and all tested organisms accumulated silver, primarily as metallic Ag0 granules within and around cell walls.

    Conclusions:

    • Yeast species can develop tolerance to silver ions through accumulation and transformation into less toxic forms.
    • Light plays a significant role in mitigating silver ion toxicity by promoting the reduction of Ag+ to Ag0.
    • The findings have implications for using yeasts in silver-based antimicrobial applications and bioremediation.