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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Published on: June 14, 2013

Respiratory oscillations in yeasts.

David Lloyd1

  • 1School of Biosciences, Cardiff University, P.O. Box 915, Cardiff CF10 3TL, Wales, UK. lloydd@cf.ac.uk

Advances in Experimental Medicine and Biology
|September 12, 2008
PubMed
Summary
This summary is machine-generated.

Yeast respiration exhibits ultradian rhythms, driven by a redox switch. These oscillations, occurring on minute timescales, coordinate cellular processes and provide a temporal framework for eukaryotic life.

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Area of Science:

  • Cellular Biology
  • Chronobiology
  • Biochemistry

Background:

  • Yeast respiration shows oscillations in three time domains: ~1 minute, ~40 minutes, and ~1 day.
  • Ultradian rhythms (~40 min) and circadian rhythms (~1 day) have been characterized, but the ~1-minute oscillations lack a demonstrated timekeeping mechanism.
  • Synchronous yeast cultures are crucial for revealing temporal structures obscured by time-averaging in asynchronous populations.

Purpose of the Study:

  • To investigate the time-structure of respiratory oscillations in yeast, particularly focusing on ultradian rhythms.
  • To elucidate the underlying mechanism of the ultradian clock in yeast.
  • To explore the broader implications of ultradian clocks for eukaryotic cellular organization.

Main Methods:

  • Utilized self-synchronized continuous cultures of Saccharomyces cerevisiae for detailed analysis.
  • Employed continuous, non-invasive real-time monitoring of oxygen uptake, carbon dioxide production, and NAD(P)H redox state.
  • Combined real-time monitoring with discrete sampling for various redox components, metabolites, and mRNA levels.

Main Results:

  • Identified a redox switch as the central component of the ultradian clock.
  • Demonstrated that ultradian oscillations optimize a plethora of cellular outputs based on a genetically determined time-base.
  • Observed ultradian-clock driven oscillations in respiration, adenylate pools, protein, RNA synthesis, and enzyme activities.

Conclusions:

  • The ultradian clock, centered on a redox switch, provides a fundamental time-base for coordinating intracellular processes and cellular structure assembly in eukaryotes.
  • This ultradian time-keeping mechanism is suggested to be a foundational element across all eukaryotic organisms.
  • The ultradian clock provides an internal temporal framework, complemented by the circadian clock for environmental synchronization.