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Plasma 65Zn kinetics in the rat.

N M Lowe¹, I Bremner, M J Jackson

¹Department of Medicine, University of Liverpool.

The British Journal of Nutrition

|May 1, 1991

Summary

This study tracks zinc-65 (65Zn) in rats, revealing it distributes between blood plasma and the liver. Zinc levels in these compartments change with dietary zinc and endotoxin exposure, suggesting a role for liver metabolism.

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Area of Science:

Biochemistry
Physiology
Toxicology

Background:

Zinc is an essential trace element crucial for numerous biological processes.
Understanding zinc kinetics and distribution is vital for assessing nutritional status and toxicological responses.
The liver plays a central role in zinc homeostasis and metabolism.

Purpose of the Study:

To investigate the pharmacokinetic behavior of zinc-65 (65Zn) after intravenous administration in rats.
To identify and characterize the physiological compartments involved in 65Zn distribution.
To examine how zinc status and endotoxin challenge affect 65Zn distribution.

Main Methods:

Intravenous injection of 65Zn in rats.
Analysis of 65Zn concentrations in plasma and liver over 90 minutes.
Two-compartment kinetic modeling to describe 65Zn distribution.
Comparative analysis under conditions of zinc depletion and endotoxin administration.

Main Results:

65Zn distribution followed two-compartment kinetics, with an initial plasma pool (Qa) and a secondary liver pool (Qb).
Plasma zinc concentration and Qa decreased during zinc depletion.
Both Qa and Qb increased following Escherichia coli endotoxin injection, despite a plasma zinc decline.
The liver pool (Qb) showed characteristics of a metabolic pool, varying with zinc status and resembling metallothionein behavior.

Conclusions:

The study elucidates the dynamic distribution of zinc between plasma and liver in rats.
Zinc homeostasis is significantly influenced by dietary zinc availability and inflammatory responses.
The liver's metabolic pool (Qb) is a key determinant of zinc distribution, responding to physiological challenges.

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