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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jan 13, 2026

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

Published on: October 5, 2018

F-Seq: a feature density estimator for high-throughput sequence tags.

Alan P Boyle¹, Justin Guinney, Gregory E Crawford

¹Institute for Genome Sciences and Policy, Duke University, Durham, NC 27708, USA.

Bioinformatics (Oxford, England)

|September 12, 2008

Summary

This summary is machine-generated.

F-Seq is a new software package that creates a continuous signal from high-throughput sequencing data. This allows for easier identification and visualization of important biological sequence features in genome browsers.

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Last Updated: Jan 13, 2026

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq

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Published on: October 5, 2018

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Area of Science:

Genomics
Bioinformatics

Background:

High-throughput sequencing technologies like ChIP-seq and DNase-seq are widely used.
Analyzing the vast amounts of sequence data generated can be challenging.

Purpose of the Study:

To develop a software package for intuitive summarization and display of individual sequence data.
To generate an accurate and interpretable signal from tag sequencing data.

Main Methods:

Developed F-Seq, a software package written in Java.
F-Seq generates a continuous tag sequence density estimation.

Main Results:

The software provides an interpretable signal from sequencing data.
Identifies biologically meaningful sites within the sequence data.

Conclusions:

F-Seq enables intuitive visualization of sequencing data.
Output is compatible with the UCSC Genome Browser for direct display.