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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jul 1, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Taking photographs with a microscope.

Richard J Maude1, Gavin C K W Koh, Kamolrat Silamut

  • 1Centre for Tropical Diseases, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford, UK. richardmaude@gmail.com

The American Journal of Tropical Medicine and Hygiene
|September 12, 2008
PubMed
Summary
This summary is machine-generated.

This study presents an easy and affordable method for capturing digital microscope images using common cameras and phones. This technique is ideal for remote diagnostics and educational purposes in resource-limited settings.

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A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software
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Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

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Area of Science:

  • Microscopy
  • Digital Imaging
  • Medical Diagnostics

Background:

  • Microscopic imaging is crucial for diagnosis and education.
  • Access to high-quality digital imaging equipment can be limited in resource-poor settings.

Purpose of the Study:

  • To present a simple, economical, and practical technique for digital photomicrography.
  • To enable image capture using readily available equipment like light microscopes, digital cameras, and cameraphones.

Main Methods:

  • Utilizing standard light microscopes with various digital cameras and cameraphones.
  • A straightforward technique adaptable to different equipment models.

Main Results:

  • The described method is quick to learn and easy to perform.
  • Successful digital image capture of specimens is achievable with common devices.

Conclusions:

  • This technique offers a practical solution for digital microscopy in resource-limited environments.
  • It supports remote diagnostics and enhances microscopy education.
  • The method's accessibility makes it valuable for global health and scientific training.