Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Allantopyrone A promotes cross-linking of ubiquitin-specific protease 5 (USP5) and inhibits its deubiquitinase activity by targeting distinct domains.

The Journal of antibiotics·2026

Same author

Kujiol A Inhibits Interferon-γ and Interleukin-2 Expression and the NFATc2 Interaction with Their Promoters in T Cells.

Molecules (Basel, Switzerland)·2026

Same author

Volumetric MRI of dorsal root ganglia as a biomarker for disease progression and response to AAV treatment in a mouse model of Fabry disease.

PloS one·2025

Same author

Furanoeremophilanes from edible wild plants belonging to the Asteraceae family inhibit ferroptosis via suppression of lipid peroxidation.

Fitoterapia·2025

Same author

Biodistribution of AAV1, AAV5, AAV9, and AAVDJ serotypes after intra-cisterna magna delivery in non-human primates.

Molecular therapy. Methods & clinical development·2025

Same author

Longitudinal insights into maternal body composition across trimesters of pregnancy.

Maternal health, neonatology and perinatology·2025

Search research articles

Related Experiment Video

Updated: Jul 1, 2026

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Published on: February 7, 2014

Chip is required for posteclosion behavior in Drosophila.

Padmanabhan Hari¹, Mugdha Deshpande, Neha Sharma

¹Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience

|September 12, 2008

Summary

The protein cofactor Chip is essential for regulating posteclosion behaviors in Drosophila by ensuring the presence of Bursicon. This study reveals Chip

More Related Videos

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Published on: December 30, 2015

Related Experiment Videos

Last Updated: Jul 1, 2026

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Published on: February 7, 2014

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Published on: December 30, 2015

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Neuronal development involves complex regulatory mechanisms controlling molecular, neurochemical, and connectional features.
Posteclosion behaviors in Drosophila, such as wing expansion and cuticle hardening, are well-characterized but their underlying developmental regulation is not fully understood.

Purpose of the Study:

To investigate the role of the protein cofactor Chip in the development of Drosophila neurons that control posteclosion behavior.
To identify the molecular mechanisms by which Chip influences these developmental processes.

Main Methods:

Utilizing multiple genetic approaches to interfere with Chip function in Drosophila.
Analyzing the effects of Chip disruption on posteclosion behaviors.
Quantifying Bursicon levels in the hemolymph of affected flies.
Investigating potential interactions between Chip and transcription factors.

Main Results:

Disruption of Chip function in specific Drosophila neurons prevents normal wing expansion and cuticle tanning after eclosion.
Flies with impaired Chip function exhibit an absence of Bursicon in their hemolymph, while retaining normal responsiveness to Bursicon.
Chip interacts with LIM-homeodomain (LIM-HD) transcription factors, with dIslet identified as a potential partner.

Conclusions:

The protein cofactor Chip plays a critical role in the development of the neuronal circuit regulating posteclosion behavior in Drosophila.
Chip is necessary for the production or release of Bursicon, a key hormone for these behaviors.
This study provides the first evidence for transcriptional mechanisms, involving Chip and dIslet, in the developmental regulation of posteclosion behavior circuits.