Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoluminescence of Rhodamine from Nano-Confinement Inside 3D Sculptured Coatings.

Nanomaterials (Basel, Switzerland)·2026
Same author

Optimal laser fluence for pulsed laser ablation synthesis of silicon nanoparticles in liquid.

Optics express·2025
Same author

On the accuracy bounds of high-order image correlation spectroscopy.

Optics express·2024
Same author

Uptake quantification of gold nanoparticles inside of cancer cells using high order image correlation spectroscopy.

Biomedical optics express·2021
Same author

Bidirectional ephrinB2‑EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells.

International journal of molecular medicine·2020
Same author

Ablation in Externally Applied Electric and Magnetic Fields.

Nanomaterials (Basel, Switzerland)·2020

Related Experiment Video

Updated: Jul 1, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Confocal reflection readout thresholds in two-photon-induced optical recording.

Xiangping Li1, James W M Chon, Min Gu

  • 1Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria 3122, Australia.

Applied Optics
|September 12, 2008
PubMed
Summary

This study investigates optical recording thresholds in photoisomerization polymers. Lowering the readout threshold is achieved through multiple reflections, enhancing image contrast for better data storage.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Related Experiment Videos

Last Updated: Jul 1, 2026

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution
08:48

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Data Storage Technologies

Background:

  • Two-photon-induced optical recording utilizes photoisomerization polymers for data storage.
  • Confocal reflection readout is a key technique for accessing stored information.
  • Understanding readout thresholds is crucial for optimizing storage density and reliability.

Purpose of the Study:

  • To theoretically and experimentally investigate confocal reflection readout thresholds in two-photon-induced optical recording.
  • To identify factors influencing readout thresholds and image quality.
  • To explore methods for reducing readout thresholds and enhancing image contrast.

Main Methods:

  • Theoretical modeling of confocal reflection readout.
  • Experimental optical recording and readout using photoisomerization polymers.
  • Analysis of axial response and image reconstruction.

Main Results:

  • A readout threshold of approximately 10(-2) was observed for planar reflectors.
  • The threshold was significantly reduced to 0.006 due to multiple reflections from recorded bits.
  • Multiple reflections enhanced image contrast and lowered the readout threshold.
  • Reconstructed bit image quality depends on refractive-index mismatch and proximity to the rare surface.

Conclusions:

  • Multiple reflections within photoisomerization polymers can drastically reduce optical readout thresholds.
  • Optimizing refractive-index mismatch and recording position is vital for high-quality optical data storage.
  • This research offers insights into improving the performance of optical recording systems.