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Updated: Jul 1, 2026
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
[Effects of Porphyromonas gingivalis on inflammatory factor secreted by human umbilical vein endothelial cells]
Xiao-yan Wang1, Jun-lian Wang, Ao Ding
1School of Stomatology, The Fourth Military Medical University, Xi'an 710032, Shanxi province, China.
Purpose:
To investigate the effect of porphyromonas gingivalis (P.g) on interleukin-18 (IL-18) and CRP secretion in human umbilical vein endothelial cells (HUVECs).
Methods:
Anaeropack method was used for P.g cultivation and then HUVECs were co-cultured with P.g followed by assaying the amount of IL-18 and CRP with enzyme-linked immunosorbent assay (ELISA) kit. Linear regression and variance analysis were performed with SPSS11.0 software package.
Results:
The unstimulated HUVECs expressed small amounts of IL-18 and CRP, P.g dose-dependently increased the production of IL-18 and CRP in HUVECs. Elevated IL-18 and CRP were found at 4-hour, then continued to increase at 8-hour, 12-hour and 24-hour after co-cultivation; under the effect of same concentration, the quantity of CRP was significantly higher than that of IL-18 secreted by HUVECs. There was significant different, P<0.05.
Conclusion:
It is concluded that P.g increased the production of IL-18 and CRP in HUVECs in a dose and time dependent manner, elevated IL-18 and CRP may be involved in the regulation of inflammatory both in periodontal disease and formation of atherosclerotic plaque in cardiovascular disease(CVD).
Insights
Porphyromonas gingivalis (P.g) significantly increases interleukin-18 (IL-18) and C-reactive protein (CRP) in human umbilical vein endothelial cells (HUVECs). This suggests a role for P.g in inflammatory conditions like periodontal disease and cardiovascular disease.
Area of Science:
- Endothelial cell biology
- Microbiology
- Immunology
Background:
- Porphyromonas gingivalis (P.g) is a key pathogen in periodontal disease.
- Interleukin-18 (IL-18) and C-reactive protein (CRP) are inflammatory markers.
- Endothelial cells play a crucial role in vascular health and inflammation.
Purpose of the Study:
- To investigate the impact of P.g on IL-18 and CRP secretion in HUVECs.
- To determine the dose and time-dependent effects of P.g on endothelial inflammatory markers.
Main Methods:
- P.g was cultured using the Anaeropack method.
- HUVECs were co-cultured with P.g.
- IL-18 and CRP levels were quantified using ELISA.
- Statistical analysis included linear regression and variance analysis.
Main Results:
- Unstimulated HUVECs produced low levels of IL-18 and CRP.
- P.g dose-dependently increased IL-18 and CRP production in HUVECs.
- Elevated IL-18 and CRP levels were observed from 4 to 24 hours post-co-cultivation.
- CRP levels were significantly higher than IL-18 levels at equivalent concentrations.
Conclusions:
- P.g stimulates IL-18 and CRP production in HUVECs in a dose- and time-dependent manner.
- Elevated IL-18 and CRP may contribute to inflammation in periodontal disease.
- These findings suggest a potential link between P.g, IL-18, CRP, and atherosclerotic plaque formation in cardiovascular disease.
