Related Experiment Video
Updated: Jul 1, 2026
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
[Radiological and histopathological changes of the periapical lesions induced by lipopolysaccharide in rats]
Qing-shan Wang1, Yan Gao, Li Rong
1Department of Endodontics, College of Stomatology, Binzhou Medical College, Binzhou 256603, Shandong Province, China. wqsyahao@yahoo.com.cn
Purpose:
To investigate histological and X-ray dynamic changes of periapical lesions induced by lipopolysaccharide(LPS) in rats.
Methods:
Experimental periapical periodontitis models in 24 Wistar rats were established by exposing pulps, placing absorbent cotton with LPS, and exposing in the oral environment. Four experimental animals were sacrificed on the day 0,3,7,14,21, and 35, respectively. Root apical radiographs and tissue sections were taken in all the teeth combined with bone from sacrificed animals. Periapical histological changes and roentgenograms were observed. The results were analyzed statistically by F test and Student's t test with SPSS12.0 software package.
Results:
On the day 3, infiltration of inflammatory cells in the periapical tissue was observed. Acute inflammatory states, severe inflammatory states and chronic inflammatory states were detected at 1 week, 2-week and 3-week,respectively. Cementum hyperplasia was observed at 5-week. Increasing periapical radiolucent areas were observed after 1 week. Intervention, there were significant differences in the periapical lesion areas at the different period time(P<0.01).
Conclusions:
The procedures that induced periapical lesions in animal model established by exposing pulps of molar teeth,placing absorbent cotton with LPS, and exposing to the oral environment is similar to human natural progress of the same disease and has a shorter time duration. It is an ideal research model for periapical periodontitis.
