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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

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Related Experiment Video

Updated: Jul 1, 2026

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
06:54

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Published on: December 8, 2010

Proteomic analysis of pluripotent stem cells.

Sean C Bendall1, Aaron T Booy, Gilles Lajoie

  • 1University of Western Ontario, London, Ontario, Canada.

Current Protocols in Stem Cell Biology
|September 12, 2008
PubMed
Summary

Mass spectrometry (MS)-based proteomics identifies proteins in embryonic stem cells (ESCs). This method analyzes peptides using MALDI-TOF MS or ESI-MS/MS for molecular insights.

Area of Science:

  • Proteomics
  • Molecular and Cellular Biology
  • Biotechnology

Background:

  • Mass spectrometry (MS)-based proteomics is a key technique for protein identification.
  • Traditional methods involve gel electrophoresis, protease digestion, and MS analysis.
  • Genomic databases are used for comparing experimental mass data.

Purpose of the Study:

  • To provide protocols for MS-based proteomics in embryonic stem cells (ESCs).
  • To enable rapid insights into molecular and cellular biology of ESCs.

Main Methods:

  • Protein isolation via one- or two-dimensional gel electrophoresis.
  • Protease digestion of isolated proteins into peptides.
  • Analysis using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or electrospray ionization tandem MS (ESI-MS/MS).

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Related Experiment Videos

Last Updated: Jul 1, 2026

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
06:54

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Published on: December 8, 2010

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

  • Comparison of peptide mass data against theoretical databases.

    • Main Results:

    • Detailed protocols for MS-based proteomics in ESCs are presented.
    • The methods allow for the identification of expressed proteins.

    Conclusions:

    • MS-based proteomics offers powerful insights into ESC molecular and cellular biology.
    • Standardized protocols facilitate reproducible protein identification in ESC research.