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Updated: Jul 1, 2026

Teratoma Generation in the Testis Capsule
Published on: November 7, 2011
Isolation of human embryonic stem cell-derived teratomas for the assessment of pluripotency
Karin Gertow1, Stefan Przyborski, Jeanne F Loring
1Department of Laboratory Medicine, Clinical Research Center, Unit for Molecular Embryology, Karolinska Institute, Sweden.
Abstract:
This unit describes protocols on how to assess the developmental potency of human embryonic stem cells (hESCs) by performing xenografting into immunodeficient mice to induce teratoma formation. hESCs can be injected under the testis capsule, or alternatively into the kidney or subcutaneously. Teratomas that develop from grafted hESCs are surgically removed, fixed in formaldehyde, and paraffin embedded. The tissues in the teratoma are analyzed histologically to determine whether the hESCs are pluripotent and form tissues derived from of all three embryonic germ layers (ectoderm, mesoderm, and endoderm). Teratomas can also be fixed in Bouin's or cryosectioned for analysis, and they can be analyzed by immunohistochemistry for tissue markers. Methods for these procedures are included in this unit.
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