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Related Experiment Video

Updated: Jul 1, 2026

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
05:31

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

Isolation of amniotic epithelial stem cells.

Toshio Miki1, Fabio Marongiu, Ewa Ellis

  • 1University of Pittsburgh, Department of Pathology, Pittsburgh, Pennsylvania, USA.

Current Protocols in Stem Cell Biology
|September 12, 2008
PubMed
Summary

Researchers developed a simple protocol to isolate amniotic epithelial cells from human placenta. This method enriches for SSEA-4 positive stem cells, offering potential for regenerative medicine applications.

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Placental tissue engineering

Background:

  • Placental stem cells offer phenotypic plasticity for regenerative medicine.
  • Existing isolation protocols can be complex or disruptive.
  • Selective isolation of specific placental cell types is needed.

Purpose of the Study:

  • To describe a simple protocol for selective isolation of amniotic epithelial cells.
  • To avoid disturbing the placental mesenchymal layer during isolation.
  • To enrich for SSEA-4 positive cells using density separation.

Main Methods:

  • Selective isolation of amniotic epithelial cells from human term placenta.
  • Density separation technique for cell enrichment.
  • Protocol designed to preserve the mesenchymal layer.

More Related Videos

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

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Last Updated: Jul 1, 2026

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
05:31

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

Published on: December 21, 2014

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Main Results:

  • A detailed and simple protocol for selective isolation of amniotic epithelial cells was established.
  • The protocol successfully isolates cells without disturbing the mesenchymal layer.
  • Density separation effectively enriches for SSEA-4 positive cells.

Conclusions:

  • The described protocol provides a straightforward method for isolating placental stem cells.
  • This technique facilitates the enrichment of SSEA-4 positive amniotic epithelial cells.
  • The isolated cells hold promise for future regenerative medicine therapies.