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Updated: Jul 1, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Occurrence of fungi and mycotoxins in corn silage, Jalisco State, Mexico
Waldina Patricia Reyes-Velázquez1, Victor Hugo Isaías Espinoza, Federico Rojo
1Laboratorio de Micotoxicología (Departamento de Salud Pública), Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), Universidad de Guadalajara (UDG), Jalisco, México. waldinar@cucba.udg.mx
Abstract:
The aim of this study was to evaluate the chemical composition, mold count and mycotoxin contamination of corn silage collected during a six month-period. The results indicated that the chemical composition and the physicochemical parameters evaluated did not show significant variation during the sampling time. Fungal count on RBC ranged from 1.7 x 10(3) to 9 x 10e8 CFU/g. Mucor, Penicillium and Aspergillus spp. were the most frequent fungal species in the corn silage. Fusarium count ranged from 1.6 x 10(3) to 1.6 x 10e8 CFU/g in Nash Snyder culture media. Aflatoxin B, fumonisins, ochratoxin A, ochratoxin B, deoxynivalenol, and zearalenone were detected throughout the period of corn silage maintenance (100% positive samples). However, only deoxynivalenol levels were higher than the maximum limit recommended by the FDA.
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