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c-Lysozyme from Senegalese sole (Solea senegalensis): cDNA cloning and expression pattern
M Alejandra Fernández-Trujillo1, Javier Porta, Manuel Manchado
1Department of Genetics, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
Fish & Shellfish Immunology
|September 13, 2008
Summary
Researchers cloned and characterized a c-type lysozyme gene from Senegalese sole. This innate immunity molecule showed increased expression in hematopoietic organs when stimulated, suggesting potential for fish defense enhancement.
Area of Science:
- Aquaculture
- Immunology
- Molecular Biology
Background:
- Lysozymes are crucial innate immunity components in fish, exhibiting significant bactericidal activity.
- Their potential as tools for enhancing fish immune defenses makes them attractive for aquaculture.
Purpose of the Study:
- To clone and characterize the full-length c-type lysozyme cDNA from Senegalese sole (Solea senegalensis).
- To investigate the expression patterns of this lysozyme under different conditions.
Main Methods:
- Rapid Amplification of Complementary DNA Ends Polymerase Chain Reaction (RACE-PCR) was used to obtain cDNA fragments.
- Sequence analysis identified catalytic and conserved residues, and pairwise alignments were performed.
Main Results:
- A 631bp cDNA sequence encoding 143 amino acids was obtained, showing high identity with other flatfish c-type lysozymes.
- Basal expression levels were observed, with significant upregulation in hematopoietic organs following stimulation with lipopolysaccharide (LPS) or infection with Photobacterium damselae.
Conclusions:
- This study provides foundational genetic and functional insights into Senegalese sole c-lysozyme.
- Further research on g-DNA structure, allelic variability, and antibacterial activity is required for biotechnological applications.
