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Updated: Jul 1, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Synthesis of photo-responsive acridine-modified DNA and its application to site-selective RNA scission
Keita Tanaka1, Yoji Yamamoto, Akinori Kuzuya
1Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan.
Abstract:
Photo-responsive phosphoramidite monomers, which bear an azobenzene between acridine and the phosphoramidite unit, were synthesized, and incorporated into oligonucleotides. Upon UV irradiation, the azobenzene in the modified DNA efficiently isomerized from the trans isomer into the cis isomer. Although the T(m) values of their duplexes with complementary DNA were not much changed by the isomerization, site-selective RNA scission was significantly accelerated by the UV irradiation when Mn(II) ion was used as the catalyst for RNA scission.
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