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High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

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Updated: Jul 1, 2026

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Automated modular preparative HPLC-MS purification laboratory with enhanced efficiency.

Oliver Guth¹, Dietmar Krewer, Björn Freudenberg

¹Bayer CropScience AG, D-40789 Monheim, Germany.

Journal of Combinatorial Chemistry

|September 16, 2008

Summary

A new high-throughput purification system enhances chemical synthesis efficiency by automating sample preparation and fractionation. This advanced laboratory setup accelerates lead optimization in drug discovery.

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Area of Science:

Chemical Synthesis
Analytical Chemistry
Drug Discovery

Background:

Automated parallel synthesis is established for productivity.
Purification of crude products is a significant bottleneck in chemical synthesis.
Lead optimization requires efficient purification of synthesized compounds.

Purpose of the Study:

To enhance the efficiency of the lead optimization process.
To establish a high-throughput purification laboratory for chemical synthesis.
To implement novel technologies for accelerated compound purification.

Main Methods:

Setup of a high-throughput High-Performance Liquid Chromatography/Mass Spectrometry (HPLC/MS) laboratory.
Utilized Covaris technology for HPLC sample preparation.
Implemented automated aliquotation, novel evaporation, and freeze-drying techniques.

Main Results:

Enabled purification of up to 48 crude products daily (200-400 mg each).
Successfully integrated Covaris technology, automated aliquotation, and freeze-drying for the first time.
Developed a comprehensive IT landscape to support the entire purification workflow.

Conclusions:

The established high-throughput HPLC/MS laboratory significantly enhances purification efficiency.
Novel integrated technologies and IT support streamline the lead optimization process.
This system provides a robust solution for accelerating drug discovery and development.