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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jul 1, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09:29

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Resolving sub-diffraction limit encounters in nanoparticle tracking using live cell plasmon coupling microscopy.

Guoxin Rong1, Hongyun Wang, Lynell R Skewis

  • 1Department of Chemistry and The Photonics Center, Boston University, Boston, Massachusetts 02215, USA.

Nano Letters
|September 16, 2008
PubMed
Summary
This summary is machine-generated.

We developed a new microscopy technique using gold nanoparticle plasmon coupling to track nanoscale distances in live cells, significantly improving resolution beyond optical limits.

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Last Updated: Jul 1, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
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Area of Science:

  • Biophysics
  • Nanotechnology
  • Cell Biology

Background:

  • Optical microscopy is limited in resolving nanoscale distances.
  • Tracking molecular interactions in live cells requires high spatial resolution.

Purpose of the Study:

  • To develop and apply a novel microscopy method for subdiffraction limit distance measurements.
  • To monitor nanoparticle interactions within live cells at unprecedented resolution.

Main Methods:

  • Utilized plasmon coupling between individual gold nanoparticle labels.
  • Employed a ratiometric detection scheme for enhanced sensitivity.
  • Applied plasmon coupling microscopy to live HeLa cells.

Main Results:

  • Achieved resolution improvement of over an order of magnitude beyond the optical diffraction limit (~500 nm).
  • Successfully resolved interparticle separations of gold nanoparticle-labeled fibronectin-integrin complexes.
  • Monitored nanoparticle encounters in real-time within living cells.

Conclusions:

  • Plasmon coupling microscopy offers a powerful tool for nanoscale biophysical measurements in live cells.
  • This technique enables the study of molecular complex dynamics at the single-particle level.
  • The method significantly enhances the resolving power of conventional optical microscopes.