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Related Experiment Video

Updated: Jul 1, 2026

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Published on: January 7, 2019

Nanofiber enabled layer-by-layer approach toward three-dimensional tissue formation.

Xiaochuan Yang¹, Jeckin D Shah, Hongjun Wang

¹Department of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology , Hoboken, New Jersey, USA.

Tissue Engineering. Part A

|September 16, 2008

Summary

This summary is machine-generated.

This study introduces a novel method for creating multilayered 3D tissues using electrospun nanofibers. This technique enables rapid, on-site cell assembly for fabricating functional dermal and skin tissues within a week.

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Published on: October 3, 2014

Related Experiment Videos

Last Updated: Jul 1, 2026

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Published on: January 7, 2019

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

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Published on: September 22, 2015

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Area of Science:

Biomaterials Engineering
Tissue Engineering
Regenerative Medicine

Background:

Functional tissue formation is crucial for regenerative medicine.
Existing methods for creating 3D tissues face challenges in efficiency and structural complexity.

Purpose of the Study:

To develop a novel, generic approach for rapid formation of multilayered 3D tissues using nanofibers.
To demonstrate the potential for creating functional dermal and skin tissues.

Main Methods:

Electrospinning of a 3:1 poly(epsilon-caprolactone) (PCL)/collagen solution into nanofibers (average diameter 454.5 +/- 84.9 nm).
On-site, layer-by-layer assembly of human dermal fibroblasts (NHDF) and PCL/collagen nanofibers.
Culture of fibroblast/fiber constructs and keratinocyte/fibroblast/fiber constructs.

Main Results:

High initial cell adhesion (88.1 +/- 1.5%) and rapid cell spreading on nanofibers.
Formation of 3D multilayered constructs with uniform cell distribution and retained cells.
Successful fabrication of dermal-like tissues and bilayer skin tissues within one week.

Conclusions:

A novel bottom-up, on-site layer-by-layer cell assembly approach using electrospinning for 3D tissue formation is reported.
This method facilitates the creation of functional tissues with multiple cell types and customized microenvironments.
The approach shows significant potential for advancing tissue engineering and regenerative medicine applications.