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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Published on: September 25, 2018
Modified linker-PCR primers facilitate complete sequencing of DGGE DNA fragments
Louise A O'Sullivan1, Gordon Webster, John C Fry
1Cardiff School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, Wales, UK. OsullivanLA@Cardiff.ac.uk
Abstract:
Modified linker-PCR primers were developed to enable complete sequencing of a DGGE band in one reaction. Commonly used bacterial and archaeal 16S rRNA gene PCR-DGGE primers were modified to contain linkers and sequencing primers. This protocol does not involve additional stages, and improves retrieval of sequence from DGGE bands by approximately 23%.
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