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Updated: Jul 1, 2026
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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
Published on: May 22, 2018
Current approaches for global post-translational modification discovery and mass spectrometric analysis
Michael D Hoffman1, Matthew J Sniatynski, Juergen Kast
1The Biomedical Research Centre, University of British Columbia, Vancouver, BC, Canada.
Analytica Chimica Acta
|September 16, 2008
Summary
Researchers are developing new multiplexed methods to analyze protein post-translational modifications (PTMs). These advanced techniques, including fluorescent, immuno-based, and mass spectrometry approaches, offer comprehensive PTM monitoring for better cellular biology understanding.
Area of Science:
- Proteomics
- Molecular Biology
- Biochemistry
Background:
- Post-translational modifications (PTMs) are crucial for cellular function.
- Hundreds of PTMs exist, making comprehensive analysis challenging.
- Traditional methods are insufficient for thorough PTM monitoring.
Purpose of the Study:
- To review recent advancements in multiplexed PTM analysis.
- To highlight novel techniques for comprehensive protein modification monitoring.
- To assess the potential of new methods for biological research.
Main Methods:
- Fluorescent stain and immuno-based methods.
- Hardware-based mass spectrometric techniques.
- Computational-based mass spectrometric approaches.
Main Results:
- Novel multiplexed methods offer enhanced PTM analysis capabilities.
- These techniques provide a more comprehensive view of protein modifications.
- Recent developments show significant promise for the field.
Conclusions:
- Advanced multiplexed PTM analysis methods are emerging.
- These methods are essential for a deeper understanding of cellular biology.
- The reviewed techniques represent valuable future resources for researchers.
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