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Updated: Jul 1, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
Published on: April 26, 2024
Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
Beatriz Maestro1, Isabel Velasco, Isabel Castillejo
1Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Avda. Universidad s/n, 03202 Elche (Alicante), Spain.
Abstract:
We present a novel procedure for affinity partitioning of recombinant proteins fused to the choline-binding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity.
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