Solid-phase extraction of fluoroquinolones from aqueous samples using a water-compatible stochiometrically imprinted
Elena Benito-Peña1, Javier L Urraca, Börje Sellergren
1Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
Journal of Chromatography. A
|September 16, 2008
Summary
A new molecularly imprinted polymer solid-phase extraction (MISPE) method selectively isolates fluoroquinolone antibiotics from water. This technique offers sensitive detection of these crucial environmental contaminants.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Polymer Science
Background:
- Fluoroquinolone antibiotics are widely used, leading to their presence in environmental water bodies.
- Effective methods for detecting and quantifying these contaminants at low concentrations are essential for environmental monitoring.
- Existing methods may lack selectivity or require complex sample preparation.
Purpose of the Study:
- To develop a novel and simple molecularly imprinted polymer solid-phase extraction (MISPE) method for selective fluoroquinolone antibiotic analysis.
- To optimize the MISPE procedure for efficient preconcentration and cleanup of target analytes from environmental water samples.
- To evaluate the performance of the developed method in terms of selectivity, recovery, precision, and detection limits.
Main Methods:
- A molecularly imprinted polymer (MIP) was synthesized using enrofloxacin as the template molecule and a urea-based functional monomer.
- The MIP material was characterized using nitrogen adsorption-desorption isotherms and scanning electron microscopy.
- The MISPE procedure was optimized by evaluating various parameters, followed by HPLC analysis with fluorescence detection (FLD).
Main Results:
- The optimized MISPE/HPLC-FLD method demonstrated good recoveries (66-100%) for danofloxacin, enrofloxacin, oxolinic acid, and flumequine.
- Moderate recoveries (15-40%) were achieved for norfloxacin, ciprofloxacin, lomefloxacin, and sarafloxacin.
- Method detection limits ranged from 0.01 to 0.30 µg/L for fluoroquinolones in river water samples processed at the ng/L level.
Conclusions:
- The developed MISPE method provides a selective and efficient approach for the preconcentration of fluoroquinolone antibiotics from environmental water samples.
- The method is capable of detecting fluoroquinolones at low ng/L levels, making it suitable for environmental monitoring.
- This novel technique offers a valuable tool for assessing the presence and impact of fluoroquinolone contamination in aquatic ecosystems.
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