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RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling
Published on: April 11, 2015
Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations
Simon McIlroy1, Kate Porter, Robert J Seviour
1Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
Applied and Environmental Microbiology
|September 16, 2008
Abstract:
We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.
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