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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Published on: January 8, 2015
A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Genetics
|September 16, 2008
Abstract:
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
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