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Suction blisters technique in amikacin diffusion through interstitial fluid in cystic fibrosis
L Vaillant1, E Autret, S Marchand
1Department of Dermatology, CHU Trousseau, Tours, France.
Insights
This study investigated amikacin tissue diffusion in children with cystic fibrosis using suction blister fluid (SBF). Results indicate good amikacin penetration and tissue diffusion, validating the SBF method for drug assessment.
Area of Science:
- Pharmacology
- Pharmacokinetics
- Pediatrics
Background:
- Aminoglycoside pharmacokinetics are altered in cystic fibrosis patients.
- Understanding drug tissue penetration is crucial for effective treatment in cystic fibrosis.
Purpose of the Study:
- To evaluate amikacin tissue diffusion in pediatric cystic fibrosis patients.
- To assess the utility of suction blister fluid (SBF) for studying drug pharmacokinetics.
Main Methods:
- Intravenous amikacin administration (5 mg/kg single dose) in two pediatric cystic fibrosis patients.
- Collection and analysis of suction blister fluid (SBF) and plasma concentrations.
- Calculation of the area under the curve (AUC) ratio between SBF and plasma.
Main Results:
- Peak plasma amikacin concentrations ranged from 11.2 to 24.5 mg/l.
- Peak SBF amikacin concentrations ranged from 3.2 to 8.3 mg/l.
- The SBF to plasma AUC ratio was 58-60%, indicating substantial tissue penetration.
Conclusions:
- Amikacin demonstrates good penetration into tissue, as evidenced by SBF concentrations.
- The suction blister fluid method shows promise for assessing drug tissue diffusion in cystic fibrosis.
- Further studies are warranted to confirm the utility of SBF in pharmacokinetic assessments.
Abstract:
Modifications in the pharmacokinetics of aminoglycosides have been reported among patients with cystic fibrosis. We obtained suction blister fluid (SBF) to study the tissue diffusion of amikacin in cystic fibrosis in 2 children (a 9-year-old boy and a 7-year-old girl) over the course of bronchopulmonary infection. Amikacin was administered intravenously using a single dose of 5 mg/kg. The peak plasma concentrations were 24.5 and 11.2 mg/l, and the peak SBF concentrations were 8.3 and 3.2 mg/l. The ratio of the area under the curve in SBF and plasma was 58 and 60%, respectively. These results suggest a good penetration of amikacin through SBF and a good tissue diffusion of amikacin. The preliminary data suggest that the suction blister method may be useful to assess the tissue diffusion of drugs.