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Updated: Jul 1, 2026

Induction and Validation of Cellular Senescence in Primary Human Cells
Published on: June 20, 2018
Coordinate expression of the human pregnancy-specific glycoprotein gene family during induced and replicative
Morio Endoh1, Yusuke Kobayashi, Yoshimi Yamakami
1International Graduate School of Arts and Sciences, Yokohama City University, Seto 22-2, Kanazawa-ku, Yokohama, Kanagawa, 236-0027, Japan.
Abstract:
Pregnancy-specific glycoproteins (PSGs) comprise a family of highly similar polypeptides encoded by 11 transcriptionally active genes that compactly cluster on band 19q13.2. All members of the PSG family were found to be markedly up-regulated by addition of 5-bromodeoxyuridine in HeLa cells. Similarly, all of the members were markedly up-regulated during replicative senescence in normal human fibroblasts. Promoter analysis of the PSG1, 4, and 11 genes in HeLa cells did not reveal a cis-regulatory element responsive to 5-bromodeoxyuridine in their 5'-flanking sequences. These results suggest that the PSG genes are regulated at a level of higher order chromatin structure besides by a signal of pregnancy.
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