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Updated: Jul 1, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
The diverse roles of protein kinase C in pancreatic beta-cell function
Trevor J Biden1, Carsten Schmitz-Peiffer, James G Burchfield
1Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, NSW 2010, Australia. t.biden@garvan.org.au
Abstract:
Members of the serine/threonine PKC (protein kinase C) family perform diverse functions in multiple cell types. All members of the family are activated in signalling cascades triggered by occupation of cell surface receptors, but the cPKC (conventional PKC) and nPKC (novel PKC) isoforms are also responsive to fatty acid metabolites. PKC isoforms are involved in various aspects of pancreatic beta-cell function, including cell proliferation, differentiation and death, as well as regulation of secretion in response to glucose and muscarinic receptor agonists. Recently, the nPKC isoform, PKCepsilon, has also been implicated in the loss of insulin secretory responsiveness that underpins the development of Type 2 diabetes.
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