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Updated: Jul 1, 2026
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Nicotine combined with okadaic acid or taxol adversely affects bovine oocyte maturation and subsequent embryo
Guang-Peng Li1, Shufen Yang, Ying Liu
1Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, People's Republic of China. guangpengli@yahoo.com
Objective:
To investigate the effects of nicotine in combination with okadaic acid (OA) or taxol on bovine oocyte maturation and subsequent embryonic development.
Design:
Prospective randomized study.
Setting:
University research laboratory.
Patient(S):
Bovine ovaries, oocytes, and embryos.
Intervention(S):
Oocyte maturation and subsequent embryo development in the presence of nicotine in combination with okadaic acid and taxol.
Main Outcome Measure(S):
Haploid composition, cleavage rate, IVF and parthenogenetic embryo development, blastocyst cell number.
Result(S):
The results showed that nicotine and OA or nicotine and taxol significantly decreased oocyte maturation rates. Combinations of nicotine (10 or 50 micromol/L) and OA (0.01 or 0.05 micromol/L) did not affect oocyte haploid composition; however, taxol alone or combined with nicotine caused a decrease in haploid composition compared with control. Parthenogenetic activation of oocytes that were matured in nicotine, OA, or taxol resulted in blastocyst development rates of 12%-17%, which were not different from the control. Various combinations of nicotine and OA or nicotine and taxol significantly lowered (3%-8%) blastocyst development compared with control. The average cell number of blastocysts derived from nicotine + OA- and nicotine + taxol-treated oocytes was lower than all other treatment groups and control. For oocytes fertilized in vitro, oocytes matured in OA, taxol, or combinations of nicotine and OA or taxol-containing media resulted in significantly lower cleavage rates and blastocyst development compared with the control group and the 10 micromol/L nicotine treatment group. The IVF embryos cultured in nicotine + OA-containing medium had a significantly lower blastocyst development rate.
Conclusion(S):
Combinations of nicotine with OA or taxol adversely affect oocyte maturation and subsequently result in poor blastocyst development.
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