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Inhibition of mitochondrial cholesterol side-chain cleavage by structural analogs of cholesterol sulfate
D G Robertson1, D Perry, J D Lambeth
1Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322.
Endocrine Research
|January 1, 1991
Abstract:
Cholesterol sulfate inhibits cholesterol side-chain cleavage in adrenal mitochondria. In this study, analogs of cholesterol sulfate were evaluated for their ability to inhibit steroidogenesis. Structural requirements for inhibitory activity included a planar A-B ring junction, an intact side chain, and a 3 beta-ester group containing a single negative charge. This structural specificity argues against cholesterol sulfate acting solely as a membrane perturbing agent or a detergent, and also differs in some features from the specificity for binding to cytochrome P-450scc.