Interaction force measurements for the design of tissue adhesives

Synthesis of tissue adhesives had been carried out in various laboratories in the past decades but the development is currently stalled. One of the key reasons, it is believed, is that researchers have not fully understood and resolved the role of the functional groups that are responsible for good adhesion to biological tissues. Further progress in synthesis is significantly hindered without this fundamental understanding. With this aim in mind, atomic force microscopy (AFM) has been exploited in this work to study the interactions between functional groups that are common to biological tissues. In this work, the AFM tip and substrates were functionalized and used to measure the non-specific interaction among these common functional groups. The ultimate aim of the study is to calculate the interaction force between a single pair of functional groups. A novel calculation method based on the AFM data and probe geometry is presented. The results provide insights into the strength of the bond between different functional groups and the could serve as a guide in selecting the appropriate functional groups in tissue adhesive synthesis. This method could be further applied to studies involving interfaces of biomedical devices where intermolecular interactions are of concern.