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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jul 1, 2026

Whole-cell Patch-clamp Recordings in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

Published on: June 15, 2016

A reliable whole cell clamp technique.

¹Key Laboratory of Molecular Biophysics of Ministry Education of China, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China. lichhhust@yahoo.com

Advances in Physiology Education

|September 17, 2008

Summary

This summary is machine-generated.

A novel syringe-based technique simplifies whole cell formation for patch-clamp experiments. This method enhances success rates and reduces labor for both novice and experienced researchers.

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Last Updated: Jul 1, 2026

Whole-cell Patch-clamp Recordings in Brain Slices

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Published on: June 15, 2016

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

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Area of Science:

Biophysics
Neuroscience
Cellular Biology

Background:

The patch-clamp technique is crucial for studying ion channels and cellular electrophysiology.
Traditional whole-cell formation methods can be challenging, requiring significant expertise and leading to variable success rates.

Purpose of the Study:

To introduce a simplified, invented technique for whole-cell formation.
To improve the ease of learning and success rate of patch-clamp experiments for researchers of all levels.

Main Methods:

Development of a novel implementation tool: a syringe with a precisely designed hole and slot.
Application of this tool in teaching and experimental settings for whole-cell configuration.

Main Results:

The invented syringe technique significantly shortens the learning curve for novices.
Experienced researchers observe an increased success rate and improved quality of whole-cell preparations.
The technique reduces experimental labor and enhances overall efficiency.

Conclusions:

The developed whole-cell formation technique offers a practical and effective solution for patch-clamp experiments.
The simplicity and improved outcomes suggest potential for broader adoption in research and education.
An automated implementation concept is proposed for future development.